CUSTOMIZATIONTailored to your needs
At Abcalis® we understand your needs. Our recombinant antibody services provide highest quality, individual customization & adaptability:
- Modular structure – our recombinant antibody services are always tailored to your specific question
- Minimize your economic risk: combine your projects from the workpackages that you need, and pay only the services provided.
- We guarantee: all workpackages of your project are done in Germany and all data are processed locally.
- Future-proof animal free / vegan antibodies: already today, our antibodies meet all criteria of EU commission document JRC120199
Assemble your project from various modules:
Target antigen design, procurement and quality control
Design of panning strategies tailored to deliver antibodies with the desired properties. Selection of antibodies in several rounds of phage display panning
Screening ELISA in scFv format (other assays and formats on request)
Sequencing, annotation and in silico analysis to identify promising candidates
Cloning into full IgG format of your choice, choose your Fc species, many other formats available as well
Antibody production in HEK cell system, choose from various purification and characterisation options
SERVICEAntibody generation
Are you facing troubles in your assays related to reproducibility?
Do you need an antibody against a new target that simply does not yet exist?
Or do you want to replace your limited batch size animal-based antibody solution?
At Abcalis® we can generate antibodies tailored to your needs from our diverse antibody phage display libraries. We take care of all steps from selection to protein production and initial validation.
The best part about it: Your final product will be completely animal-free.
SERVICEAntibody format conversion
Do you want to convert your Hybridoma into a recombinant antibody to truly immortalize it?
Do you need one of your sequence defined antibodies with a different Fc part?
At Abcalis® we can bestow your antibody with any Fc part from any species. Additionally, we have created several special formats that may be of use to you:
- TWIN-Strep tag: antibodies that carry a TWIN-strep tag are compatible with the Strep-Tactin® purification and detection system by IBA Lifesciences.
- Free-Cysteine: antibodes are engineered to have a free cysteine in a defined position for single covalent conjugation in a defined position.
- HIS-tag: antibodies carrying a 6x histidine tag are compatible with all HIS-tag based purification and immobilization systems.
- CBM3a: antibodies that carry a cellulose binding domain can be immobilized on cellulose membranes that are biodegradable in contrast to the widely used nitro-cellulose membranes.
- CBM77 (under development): antibodies that carry a nitro-cellulose binding domain can be immobilized on nitro-cellulose membranes in a directional manner.
- HRP: HRP conjugation is a standard in-house process for our detection antibodies and can be applied to any other.
- Biotin: all antibodies can also be supplied in a biotinylated form.
Delivery of known sequence or sequencing of antibody gene
Design of DNA / gene delivery or gene synthesis
Primer design & PCR
Cloning into full IgG format of your choice
Antibody production in HEK cell system
SERVICESmall Molecule Sandwich Detection
Do you want to improve your competitive small molecule ELISA?
Abcalis provides an antibody discovery service that transforms competitive small molecule immunoassays into sandwich ELISA formats. Our approach enables the generation of highly specific antibodies that selectively recognize the target only when bound to its specific capture antibody. This innovation allows for enhanced signal amplification, leading to increased assay sensitivity and robustness.
By eliminating the constraints of competitive ELISA, our technology enables a more flexible detection method for small molecules in diagnostics and research applications.
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