Multiclonals

Abcalis® Multiclonals are a revolutionary new class of secondary antibodies

Abcalis® believes that the future of secondary antibodies is recombinant, sequence defined and animal free. In therapeutics, recombinant antibodies have long been the gold standard for real-world application and we do not see any reason why this should be different for diagnostics and research. Multiclonals combine the best of polyclonal antisera and hybridoma monoclonal antibodies, while eliminating their disadvantages, plus they add the quality of recombinant reagents.
Our Multiclonal antibodies are a mixture of different, carefully selected recombinant monoclonal immunglobulins. Their respective epitope binding sites do not compete with each other, therefore amplifying signal strength in each and every one of your test results.

Read our newly released Tech Note for all scientific details!

As is the standard with all Abcalis® products, they are all completely sequence defined, implying their unlimited long-term availability and always identical test results. Their composition of individually characterised antibodies minimizes cross-reactivity  with other targets, since they do not contain non-target directed IgG like all animal derived polyclonals do. This allows for a much lower unspecific binding reactivity in many assays compared to animal based products – see data of a direct comparison below.

This is how we make multiclonal antibodies without the need for animal immunisation or the use of other animal materials (except some milk powder for for blocking):

 

Abcalis® Multiclonals can contain up to 17 different individually tested monoclonal recombinant antibodies. This provides the typical advantage of polyepitope recognition which is key to the broad application profile of polyclonal antisera, but eliminates their disadvantages (limited batch sizes and batch-to-batch variations, no long-term reproducibility, undefined composition, unknown constituents) and allow to minimize your QC. Hence, you are receiving all the advantages of conventional polyclonal mixtures, but without their downsides.

Scroll down to see a list of our currently available Multiclonal mixtures. More specificities and labels are in the making!
All prices are in Euro and are given without german VAT (Umsatzsteuer).

Multiclonals combine the best of polyclonal, monoclonal and recombinant antibodies

multiclonals_advantages

Abcalis® Multiclonal Anti human IgG(Fc) secondary antibodies show lower cross-reactivity

Abcalis anti human Immunoglobulin multiclonals consist of carefully adjusted mixtures recognizing different epitopes on all four different subclasses of human IgG:

Direct comparison of Abcalis multiclonals with typical animal-derived products revealed a much lower cross-reactivity binding of the recombinant product in some applications. Example:

Application examples


Quantitative capillary western blots

Simple Western Immunoassay* is an automated gel-free and blot–free method to provide quantitative immunoblot results. An antigen target was separated by capillary electrophoresis according to its size, then identified by antigen specific primary human IgG. Either Abcalis® MULTICLONAL or animal derived secondary antibody anti-human IgG HRP were used to detect and visualize the reaction with a chemiluminescent substrate. The resulting chemiluminescent signal allows quantification over a broad range of concentrations.

*Simple Western Immunoassay™ and Protein Simple™ are trademarks of ProteinSimple, San Jose, USA


Multiclonal mixtures (click on name for details and price)

Recombinant IgG mix, mouse Fc, unconjugated

ABMC-001-M                                                                     100µl, unconjugated                                 request quote 

Antigen species

human Immunglobulin, Fc-part

Specificity / Activity

human Immunglobulin
(IgG1 + IgG2 + IgG3 + IgG4), Fc specific,
denatured IgG is not recognised, no cross reactivity with bovine, rabbit, goat and horse IgG

Label

none

Product format

ready to use mixture of monoclonal recombinant antibodies in PBS, recommended dilution >1:1000

Validated assay compatibility 

FACS, ELISA, Immunohistochemistry, Immunofluorescence

Epitopes

multiple, within Fc-Part

Antibody originantibody clones were obtained by phage display from a universal library, produced as IgG with mouse Fc parts in human cells, Protein A purified

Detection with: 

anti-mouse secondary antibody

Additional Information 

see Tech Note

Animal Use Statement

discovery and production animal free

Recombinant IgG mix, mouse Fc, conjugated to horseradish peroxidase

ABMC-001-M-HRP                                                            100µl, HRP conjugated                          available soon 

Antigen species

human Immunglobulin, Fc-part

Specificity / Activity

human Immunglobulin
(IgG1 + IgG2 + IgG3 + IgG4), Fc specific,
denatured IgG is not recognised, no cross reactivity with bovine, rabbit, goat and horse IgG

Label

Horseradish peroxidase (HRP)

Product format

ready to use mixture of monoclonal recombinant antibodies in PBS, recommended dilution >1:1000

Validated assay compatibility 

as secondary antibody in: FACS, ELISA, Immunoblot, Immunohistochemistry

Epitopes

multiple, within Fc-Part

Antibody originantibody clones were obtained by phage display from a universal library, produced as IgG with mouse Fc parts in human cells, Protein A purified

Detection with: 

Horseradish peroxidase (HRP) substrates

Additional Information 

see Tech Note

Animal Use Statement

discovery and production animal free

Recombinant IgG mix, mouse Fc, conjugated to alkaline phosphatase

ABMC-001-M-AP                                                               conjugated to alkaline phosphatase        available soon

Antigen species

human Immunglobulin, Fc-part

Specificity / Activity

human Immunglobulin
(IgG1 + IgG2 + IgG3 + IgG4), Fc specific,
denatured IgG is not recognised, no cross reactivity with bovine, rabbit, goat and horse IgG

Label 

E. coli Alkaline Phosphatase

Product format

ready to use mixture of monoclonal recombinant antibodies in PBS, recommended dilution >1:1000

Validated assay compatibility 

as secondary antibody in: FACS, ELISA, Immunoblot, Immunohistochemistry

Epitopes

multiple, within Fc-Part

Antibody originantibody clones were obtained by phage display from a universal library, produced as IgG with mouse Fc parts in human cells, Protein A purified

Detection with: 

Alkaline Phosphatase substrates

Additional Information 

see Tech Note

Animal Use Statement

discovery and production animal free

Recombinant IgG mix, mouse Fc, conjugated to Alexa488

ABMC-001-M-488                                      conjugated to fluorescence dye Alexa488/BODIPY      available soon

Antigen species

human Immunglobulin, Fc-part

Specificity / Activity

human Immunglobulin
(IgG1 + IgG2 + IgG3 + IgG4), Fc specific,
denatured IgG is not recognised, no cross reactivity with bovine, rabbit, goat and horse IgG

Label

Alexa488/BODIPY fluorescent dye

Product format

ready to use mixture of monoclonal recombinant antibodies in PBS, recommended dilution >1:500

Validated assay compatibility 

as secondary antibody in: FACS, Immunofluorescence

Epitopes

multiple, within Fc-Part

Antibody originantibody clones were obtained by phage display from a universal library, produced as IgG with mouse Fc parts in human cells, Protein A purified

Detection with: 

instruments compatible with the fluorescence profile of Alexa488/BODIPY (excitation maximum around 495nm, emission maximum around 520nm)

Additional Information 

see Tech Note

Animal Use Statement

discovery and production animal free