A new class of recombinant secondary antibodies.
Antibodies are a vital component in medical diagnostic tests. Especially secondary antibodies are used in a wide variety of immunodiagnostic assays and testing kits to either prove or disprove the presence of pathogenic antigens in patients.
Theres is just one problem: Today’s secondary antibodies are either polyclonal mixtures taken and purified directly from animal sera or monoclonal antibodies derived from hybridomas, both of which are of animal origin.
This generates a multitude of challenges:
- Animals are used for immunization and blood extraction through which they eventually die
- A stark fluctuation of quality due to different animal sera and individually occurring impurities
- Since every animal provides a unique composition of antibodies, the consequences are limited availability and no product continuity
Abcalis® MULTICLONALS combine the best of two worlds.
Go direct to our Multiclonal secondary antibody products here.
All Abcalis® Multiclonals are produced recombinantly and can therefore benefit from the established advantages of polyclonal mixtures, while eliminating the downsides regarding long-term availability and reproducibility, antigen purification and lack of continuous product identity.
Our antibodies are selected by 2018 Nobel-Prize Winning technology Antibody Phage Display in vitro. Our process is based on know-how and libraries developed by one of its inventors, building on the experience of >25 years.
No animals are used for their production.
Check out below why our antibodies are different from animal-derived monoclonal antibodies from hybridomas.
A recent international multicentric sequencing study showed that about one third of 185 sequenced hybridoma cell lines secreted mixtures of different IgGs with at least three different antigen binding sites, thus compromising both specificity and binding strength. Original paper:
- Bradbury, A.R.M. et al. (2018) When monoclonal antibodies are not monospecific: hybridomas frequently express additional functional variable regions. mAbs, 10, 539-546
Free download of this paper
For more reasons check out our mission.
Crystal structure of a Fab fragment against Pseudomonas PAO1 FliD generated with Abcalis® technology (Postel et al.(2016) eLIFE 5:e18857.