Monoclonals
Monoclonal antibodies with sequence-defined composition
All ABCALIS® Antibodies are made in vitro by animal-free recombinant methods (phage display).
All our antibodies are of completely defined composition, as the encoding DNA sequence of all components in known. This assures always identical results, unlimited long-term availability and therefore true reproducibility.
All antibody products are recombinantly produced in animal-serum free media by human HEK293 cell culture and purified from the supernatant by Protein A affinity chromatography.
All prices are in Euro and are given without german VAT (Umsatzsteuer).
Monoclonal recombinant antibody Hyper-Myc is an improved engineered anti-myc tag antibody. It has performed with better results in direct comparison to hybridoma antibody 9E10 (anti-c-myc-tag) that shares the same epitope. It is further thoroughly characterized by cross reactivity profiling on 28895 human epitopes showing higher specificity and increased monovalent affinity (KD= 17nM). Further, it provides the additional choice to be detected with either anti-rabbit Fc , anti-human Fc or anti-Mouse Fc secondary antibodies (See products ABC0501, ABC0502 and ABC0503.
ABC0502 (purified IgG with rabbit Fc-part) 100µg, lyophyllized € 449.-
Antigen species | Human |
Specificity / Activity | myc-tag (Sequence from oncogene c-Myc), For detailed cross reactivity study using Arrays with 28000 human antigenic epitopes see TechNote |
Affinity | Monovalent Affinity: KD 1,7 x 10e-8 |
Epitope sequence | c-myc tag peptide: EQKLISEEDL |
Validated assay compatibility | ELISA, western blot, Immunofluorescence, Microarrays, capillary immunoblot |
Epitope | EQKLISEEDL (“myc-tag”) as peptide or fusion protein, for detailed interaction study see TechNote |
| Antibody origin | Hyper-Myc is an engineered monoclonal recombinant antibody (IgG) obtained by in vitro evolution employing phage display of a synthetic gene library inspired by the published sequence of Myc1-9E10 (Fuchs et al. 1997, Hybridoma 16, 227-233). Delivered as lyophilized Protein-A purified high purity protein, free of serum components. |
Detection with: | Anti-rabbit IgG, Fc specific |
Additional Information | Additional data here: TechNote |
Animal Use Statement | No animal immunisation was involved in its generation, and its production in human cell culture is achieved without the use of fetal calf serum, BSA or other animal derived materials. The genes for the immunoglobulin constant regions of the mouse were obtained as recombinant DNA from the catalogue. |
Monoclonal recombinant antibody Hyper-Myc is an improved engineered anti-myc tag antibody. It has performed with better results in direct comparison to hybridoma antibody 9E10 (anti-c-myc-tag) that shares the same epitope. It is further thoroughly characterized by cross reactivity profiling on 28895 human epitopes showing higher specificity and increased monovalent affinity (KD= 17nM). Further, it provides the additional choice to be detected with either anti-rabbit Fc , anti-human Fc or anti-Mouse Fc secondary antibodies (See products ABC0501, ABC0502 and ABC0503.
ABC0501 (purified IgG with human Fc-part) 100µg, lyophyllized € 449.-
Antigen species | Human |
Specificity / Activity | myc-tag For detailed cross reactivity study using Arrays with 28000 human antigenic epitopes see TechNote |
Affinity | Monovalent Affinity: KD 1,7 x 10e-8 |
Epitope sequence | c-myc tag peptide: EQKLISEEDL |
Validated assay compatibility | ELISA, western blot, Immunofluorescence, Microarrays, capillary immunoblot |
Epitope | EQKLISEEDL (“myc-tag”) as peptide or fusion protein, for detailed interaction study see TechNote |
| Antibody origin | Hyper-Myc is an engineered monoclonal recombinant antibody (IgG) obtained by in vitro evolution employing phage display of a synthetic gene library inspired by the published sequence of Myc1-9E10 (Fuchs et al. 1997, Hybridoma 16, 227-233). Delivered as lyophilized Protein-A purified high purity protein, free of serum components. |
Detection with: | Anti-mouse IgG, Fc specific |
Additional Information | Additional data here: TechNote |
Animal Use Statement | No animal immunisation was involved in its generation, and its production in human cell culture is achieved without the use of fetal calf serum, BSA or other animal derived materials. The genes for the immunoglobulin constant regions of the mouse were obtained as recombinant DNA from the catalogue. |
Monoclonal recombinant antibody Hyper-Myc is an improved engineered anti-myc tag antibody. It has performed with better results in direct comparison to hybridoma antibody 9E10 (anti-c-myc-tag) that shares the same epitope. It is further thoroughly characterized by cross reactivity profiling on 28895 human epitopes showing higher specificity and increased monovalent affinity (KD= 17nM). Further, it provides the additional choice to be detected with either anti-rabbit Fc , anti-human Fc or anti-Mouse Fc secondary antibodies (See products ABC0501, ABC0502 and ABC0503.
ABC0503 (purified IgG with mouse Fc-part) 100µg, lyophyllized € 449.-
Antigen species | Human |
Specificity / Activity | myc-tag For detailed cross reactivity study using Arrays with 28000 human antigenic epitopes see TechNote |
Affinity | Monovalent Affinity: KD 1,7 x 10e-8 |
Epitope sequence | c-myc tag peptide: EQKLISEEDL |
Validated assay compatibility | ELISA, western blot, Immunofluorescence, Microarrays, capillary immunoblot |
Epitope | EQKLISEEDL (“myc-tag”) as peptide or fusion protein, for detailed interaction study see TechNote |
| Antibody origin | Hyper-Myc is an engineered monoclonal recombinant antibody (IgG) obtained by in vitro evolution employing phage display of a synthetic gene library inspired by the published sequence of Myc1-9E10 (Fuchs et al. 1997, Hybridoma 16, 227-233). Delivered as lyophilized Protein-A purified high purity protein, free of serum components. |
Detection with: | Anti-mouse IgG, Fc specific |
Additional Information | Additional data here: TechNote |
Animal Use Statement | No animal immunisation was involved in its generation, and its production in human cell culture is achieved without the use of fetal calf serum, BSA or other animal derived materials. The genes for the immunoglobulin constant regions of the mouse were obtained as recombinant DNA from the catalogue. |
ABC0214H (with human Fc-part) 100µg, lyophyllized € 312.-
ABC0214M (with mouse Fc-part) 100µg, lyophyllized request quote
ABC0214R (with rabbit Fc-part) 100µg, lyophyllized request quote
Antigen species | Human |
Specificity / Activity | Good Internalisation. Iinhibited the proliferation of CD30+ Karpas299 cells in a dose dependent and effector independent manner with an IC50 of 100 nM |
Affinity | KD 3.3 x 10e-8 |
Uniprot ID | P28908 |
Assay compatibility | reducing and non-reducing immunoblot, ELISA, FACS, Immunofluorescence |
Epitope | Extracellular domain of CD30 |
Original antibody clone | SH313-B5 |
Antibody origin | Monoclonal recombinant scFv-Fc antibody, antigen binding V regions selected from non-immunized human antibody phage display library connected to Fc part. Protein-A purified high purity protein, free of serum components |
Detection with: | Available with human Fc (mouse or rabbit on request): Detection by anti-human IgG-Fc specific secondary antibody (standard) if ordered with human Fc-part (order number ending with “H”); accordingly detection by anti-mouse IgG specific secondary antibody if ordered with mouse Fc-part (order number ending with “M”) or detection by anti-rabbit IgG specific secondary antibody if ordered with human Fc-part (order number ending with “R”). Fc of other species, or alternative formats (e.g. IgG, scFv) possible on request. |
Additional Information | Pls. find additional data in the paper cited below |
References | Wezler et al. (2012) Human antibodies targeting CD30+ lymphomas. Human Antibodies 21:13-28 |
ABC0215H (with human Fc-part) 50µg, lyophyllized € 399.-
ABC0215M (with mouse Fc-part) 50µg, lyophyllized request quote
ABC0215R (with rabbit Fc-part) 50µg, lyophyllized request quote
Antigen species | Human Immunodeficiency Virus 1 |
Specificity / Activity | immune precipitated soluble gp140 from culture supernatants. ELISA. Does not react with gp120 in ELISA. Reacted with gp41, this reaction was inhibited by prior SDS/DTT treatment |
Affinity | – |
Antigen origin | recombinant HIV-1 ADA.C1 gp140 |
Assay compatibility | ELISA, immunoblot and pull downs |
Epitope | WMEWEREIE (list of 15 other recognised /non-recognised peptide variants is given in publication) |
Original antibody clone | A2 |
Antibody origin | Monoclonal recombinant scFv-Fc antibody, antigen binding V regions selected from non-immunized human antibody phage display library connected to Fc part. Protein-A purified high purity protein, free of serum components |
Detection with: | Available with human Fc (mouse or rabbit on request): Detection by anti-human IgG-Fc specific secondary antibody (standard) if ordered with human Fc-part (order number ending with “H”); accordingly detection by anti-mouse IgG specific secondary antibody if ordered with mouse Fc-part (order number ending with “M”) or detection by anti-rabbit IgG specific secondary antibody if ordered with human Fc-part (order number ending with “R”). Fc of other species, or alternative formats (e.g. IgG, scFv) possible on request. |
Additional Information | Additional data here: Download reference |
References | Trott et al. (2014). Functional characterization of two scFv-Fc antibodies from an HIV controller selected on soluble HIV-1 Env complexes: a neutralizing V3- and a trimer-specific gp41 antibody. PLoS ONE 9: e97478 |
ABC0220H (with human Fc-part) 100µg, lyophyllized € 419.-
ABC0220M (with mouse Fc-part) 100µg, lyophyllized request quote
ABC0220R (with rabbit Fc-part) 100µg, lyophyllized request quote
Antigen species | Clostridioides difficile (former name: Clostridium difficile) |
Specificity / Activity | GTD domain of TcdB (Toxin B) |
Affinity | – |
Uniprot ID | Q189K3 |
Assay compatibility | ELISA, Western-Blot. |
Epitope | amino acids 23-83 (Epitope region in GTD domain) |
Original antibody clone | VIF090-A6 |
Antibody origin | Monoclonal recombinant scFv-Fc antibody, antigen binding V regions selected from non-immunized human antibody phage display library connected to Fc part. Protein-A purified high purity protein, free of serum components |
Detection with: | Available with human Fc (mouse or rabbit on request): Detection by anti-human IgG-Fc specific secondary antibody (standard) if ordered with human Fc-part (order number ending with “H”); accordingly detection by anti-mouse IgG specific secondary antibody if ordered with mouse Fc-part (order number ending with “M”) or detection by anti-rabbit IgG specific secondary antibody if ordered with human Fc-part (order number ending with “R”). Fc of other species, or alternative formats (e.g. IgG, scFv) possible on request. |
Additional Information | Additional data here: Download reference |
References | Fühner et al. (2018). Development of Neutralizing and Non-neutralizing Antibodies Targeting Known and Novel Epitopes of TcdB of Clostridioides difficile. Frontiers in Microbiology 9: 2908 |
ABC0221H (with human Fc-part) 100µg, lyophyllized € 389.-
ABC0221M (with mouse Fc-part) 100µg, lyophyllized request quote
ABC0221R (with rabbit Fc-part) 100µg, lyophyllized request quote
Antigen species | Clostridioides difficile (former name: Clostridium difficile) |
Specificity / Activity | GTD domain of TcdB (Toxin B). Binding in ELISA to single GT domain is weak, binding to full length toxin preferred, inhibits TcdB in vitro. |
Affinity | n.d. |
Uniprot ID | Q189K3 |
Assay compatibility | ELISA, inhibits TcdB in vitro. Does not work in Western-Blot. |
Epitope | amino acids 423-433 (Epitope region in GTD domain) |
Original antibody clone | SH1429-B1 |
Antibody origin | Monoclonal recombinant scFv-Fc antibody, antigen binding V regions selected from non-immunized human antibody phage display library connected to Fc part. Protein-A purified high purity protein, free of serum components |
Detection with: | Available with human Fc (mouse or rabbit on request): Detection by anti-human IgG-Fc specific secondary antibody (standard) if ordered with human Fc-part (order number ending with “H”); accordingly detection by anti-mouse IgG specific secondary antibody if ordered with mouse Fc-part (order number ending with “M”) or detection by anti-rabbit IgG specific secondary antibody if ordered with human Fc-part (order number ending with “R”). Fc of other species, or alternative formats (e.g. IgG, scFv) possible on request. |
Additional Information | Additional data here: Download reference |
References | Fühner et al. (2018). Development of Neutralizing and Non-neutralizing Antibodies Targeting Known and Novel Epitopes of TcdB of Clostridioides difficile. Frontiers in Microbiology 9: 2908 |
ABC0222H (with human Fc-part) 100µg, lyophyllized € 419.-
ABC0222M (with mouse Fc-part) 100µg, lyophyllized request quote
ABC0222R (with rabbit Fc-part) 100µg, lyophyllized request quote
Antigen species | Clostridioides difficile (former name: Clostridium difficile) |
Specificity / Activity | CROP (receptor binding) domain of TcdB (Toxin B) |
Affinity | n.d. |
Uniprot ID | Q189K3 |
Assay compatibility | ELISA, Western-Blot |
Epitope | amino acids 1862-1868 and 2220-2226 (DKYYFNP) |
Original antibody clone | VIF137-E7 |
Antibody origin | Monoclonal recombinant scFv-Fc antibody, antigen binding V regions selected from non-immunized human antibody phage display library connected to Fc part. Protein-A purified high purity protein, free of serum components |
Detection with: | Available with human Fc (mouse or rabbit on request): Detection by anti-human IgG-Fc specific secondary antibody (standard) if ordered with human Fc-part (order number ending with “H”); accordingly detection by anti-mouse IgG specific secondary antibody if ordered with mouse Fc-part (order number ending with “M”) or detection by anti-rabbit IgG specific secondary antibody if ordered with human Fc-part (order number ending with “R”). Fc of other species, or alternative formats (e.g. IgG, scFv) possible on request. |
Additional Information | Additional data here: Download reference |
References | Fühner et al. (2018). Development of Neutralizing and Non-neutralizing Antibodies Targeting Known and Novel Epitopes of TcdB of Clostridioides difficile. Frontiers in Microbiology 9: 2908 |
ABC0230H (with human Fc-part) 100µg, lyophyllized € 349.-
ABC0230M (with mouse Fc-part) 100µg, lyophyllized request quote
ABC0230R (with rabbit Fc-part) 100µg, lyophyllized request quote
Antigen species | Human |
Specificity / Activity | ADP-ribosylated RhoA/B (target of Clostridium botulinum ADP-ribosyltransferases exoenzyme C3). Only binds RhoA and B when ribosylated. Does not bind RhoC. |
Affinity | n.d. |
Uniprot ID | RhoA: P61586, RhoB: P62745 |
Assay compatibility | ELISA, Western-Blot |
Epitope | DQFPEVYVPTVFEnYVADIEVDGK, N14(ADP-Ribosyl) |
Original antibody clone | VIF140-A1 |
Antibody origin | Monoclonal recombinant scFv-Fc antibody, antigen binding V regions selected from non-immunized human antibody phage display library connected to Fc part. Protein-A purified high purity protein, free of serum components |
Detection with: | Available with human Fc (mouse or rabbit on request): Detection by anti-human IgG-Fc specific secondary antibody (standard) if ordered with human Fc-part (order number ending with “H”); accordingly detection by anti-mouse IgG specific secondary antibody if ordered with mouse Fc-part (order number ending with “M”) or detection by anti-rabbit IgG specific secondary antibody if ordered with human Fc-part (order number ending with “R”). Fc of other species, or alternative formats (e.g. IgG, scFv) possible on request. |
Additional Information | Additional data here: Download Reference |
References | Rohrbeck et al. (2016). Detection and Quantification of ADP-Ribosylated RhoA/B by Monoclonal Antibody. Toxins 8:100 |
ABC0231H (with human Fc-part) 100µg, lyophyllized € 378.-
ABC0231M (with mouse Fc-part) 100µg, lyophyllized request quote
ABC0231R (with rabbit Fc-part) 100µg, lyophyllized request quote
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Antigen species |
Neurospora crassa |
|
Specificity / Activity |
Hex1 (Woronin body major protein) |
|
Affinity |
n.d. |
|
Uniprot ID |
P87252 |
|
Assay compatibility |
ELISA, immuno gold labeling and electron microscopy |
|
Epitope |
MGYYDDDAHGHVEAD |
|
Original antibody clone |
SH1237-F8 |
|
Antibody origin |
Monoclonal recombinant scFv-Fc antibody, antigen binding V regions selected from non-immunized human antibody phage display library connected to Fc part. Protein-A purified high purity protein, free of serum components |
|
Detection with: |
Available with human Fc (mouse or rabbit on request): Detection by anti-human IgG-Fc specific secondary antibody (standard) if ordered with human Fc-part (order number ending with “H”); accordingly detection by anti-mouse IgG specific secondary antibody if ordered with mouse Fc-part (order number ending with “M”) or detection by anti-rabbit IgG specific secondary antibody if ordered with human Fc-part (order number ending with “R”). Fc of other species, or alternative formats (e.g. IgG, scFv) possible on request. |
|
Additional Information |
Additinal data can be found in the cited paper |
|
References |
Wirsing et al. (2015). Linear discriminant analysis identifies mitochondrially localized proteins in Neurospora crassa. Journal of Proteome Research 14: 3900-3911 |
ABC0233H (with human Fc-part) 100µg, lyophyllized € 399.-
ABC0233M (with mouse Fc-part) 100µg, lyophyllized request quote
ABC0233R (with rabbit Fc-part) 100µg, lyophyllized request quote
Antigen species | Pseudomonas aeruginosa |
Specificity / Activity | flagellar capping protein FLiD |
Affinity | n.d. |
Uniprot ID/Epitope sequence | Q9K3C5 |
Assay compatibility | ELISA, Western-Blot |
Epitope | – |
Original antibody clone | SH1579-B7 |
Antibody origin | Monoclonal recombinant scFv-Fc antibody, antigen binding V regions selected from non-immunized human antibody phage display library connected to Fc part. Protein-A purified high purity protein, free of serum components |
Detection with: | Available with human Fc (mouse or rabbit on request): Detection by anti-human IgG-Fc specific secondary antibody (standard) if ordered with human Fc-part (order number ending with “H”); accordingly detection by anti-mouse IgG specific secondary antibody if ordered with mouse Fc-part (order number ending with “M”) or detection by anti-rabbit IgG specific secondary antibody if ordered with human Fc-part (order number ending with “R”). Fc of other species, or alternative formats (e.g. IgG, scFv) possible on request. |
Additional Information | Pls. find additional data in the paper cited below |
References | Postel et al. (2016). Bacterial flagellar capping proteins adopt diverse oligomeric states. eLIFE 5:e18857
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ABC0235H (with human Fc-part) 100µg, lyophyllized € 379.-
ABC0235M (with mouse Fc-part) 100µg, lyophyllized request quote
ABC0235R (with rabbit Fc-part) 100µg, lyophyllized request quote
Antigen species | Human |
Specificity / Activity | SMAD2 |
Affinity | n.d. |
Uniprot ID | Q15796 |
Assay compatibility | ELISA, protein array, IHC, proximity ligation |
Epitope | DQQLNQSMDTGSPAELSPTTL |
Original antibody clone | PAS4-G7 |
Antibody origin | Monoclonal recombinant scFv-Fc antibody, antigen binding V regions selected from non-immunized human antibody phage display library connected to Fc part. Protein-A purified high purity protein, free of serum components |
Detection with: | Available with human Fc (mouse or rabbit on request): Detection by anti-human IgG-Fc specific secondary antibody (standard) if ordered with human Fc-part (order number ending with “H”); accordingly detection by anti-mouse IgG specific secondary antibody if ordered with mouse Fc-part (order number ending with “M”) or detection by anti-rabbit IgG specific secondary antibody if ordered with human Fc-part (order number ending with “R”). Fc of other species, or alternative formats (e.g. IgG, scFv) possible on request. |
Additional Information | Additional data here: Download Reference |
References | Blokzijll et al. (2016). Single chain antibodies as tools to study TGF-β regulated SMAD proteins in proximity ligation-based pharmacological screens. Molecular&Cellular Proteomics 15: 1848-1856 |
ABC0236H (with human Fc-part) 100µg, lyophyllized € 399.-
ABC0236M (with mouse Fc-part) 100µg, lyophyllized request quote
ABC0236R (with rabbit Fc-part) 100µg, lyophyllized request quote
Antigen species | Human |
Specificity / Activity | SMAD4 |
Affinity | n.d. |
Uniprot ID | Q13485 |
Assay compatibility | ELISA, Western-Blot, protein Array, IHC, proximity ligation |
Epitope | HPPSNRASTETYSTPALLA |
Original antibody clone | PAS7-C7 |
Antibody origin | Monoclonal recombinant scFv-Fc antibody, antigen binding V regions selected from non-immunized human antibody phage display library connected to Fc part. Protein-A purified high purity protein, free of serum components |
Detection with: | Available with human Fc (mouse or rabbit on request): Detection by anti-human IgG-Fc specific secondary antibody (standard) if ordered with human Fc-part (order number ending with “H”); accordingly detection by anti-mouse IgG specific secondary antibody if ordered with mouse Fc-part (order number ending with “M”) or detection by anti-rabbit IgG specific secondary antibody if ordered with human Fc-part (order number ending with “R”). Fc of other species, or alternative formats (e.g. IgG, scFv) possible on request. |
Additional Information | Additional data here: Download Reference |
References | Blokzijll et al. (2016). Single chain antibodies as tools to study TGF-β regulated SMAD proteins in proximity ligation-based pharmacological screens. Molecular&Cellular Proteomics 15: 1848-1856 |
ABC0240H (with human Fc-part) 100µg, lyophyllized € 389.-
ABC0240M (with mouse Fc-part) 100µg, lyophyllized request quote
ABC0240R (with rabbit Fc-part) 100µg, lyophyllized request quote
Antigen species | Human |
Specificity / Activity | SMAD7 |
Affinity | n.d. |
Uniprot ID | O15105 |
Assay compatibility | ELISA, IHC, proximity ligation |
Epitope | KAFDYEKAYSLQRPNDHE |
Original antibody clone | SH585-IIC4 |
Antibody origin | Monoclonal recombinant scFv-Fc antibody, antigen binding V regions selected from non-immunized human antibody phage display library connected to Fc part. Protein-A purified high purity protein, free of serum components |
Detection with: | Available with human Fc (mouse or rabbit on request): Detection by anti-human IgG-Fc specific secondary antibody (standard) if ordered with human Fc-part (order number ending with “H”); accordingly detection by anti-mouse IgG specific secondary antibody if ordered with mouse Fc-part (order number ending with “M”) or detection by anti-rabbit IgG specific secondary antibody if ordered with human Fc-part (order number ending with “R”). Fc of other species, or alternative formats (e.g. IgG, scFv) possible on request. |
Additional Information | Additional data here: Download Reference |
References | Blokzijll et al. (2016). Single chain antibodies as tools to study TGF-β regulated SMAD proteins in proximity ligation-based pharmacological screens. Molecular&Cellular Proteomics 15: 1848-1856 |
ABC0202H (with human Fc-part) 100µg, lyophyllized € 387.-
ABC0202M (with mouse Fc-part) 100µg, lyophyllized request quote
ABC0202R (with rabbit Fc-part) 100µg, lyophyllized request quote
Antigen species | Danio rerio (Zebrafish) |
Specificity / Activity | Specificity verified on cadherin knock downs and by co-location with cadherin-GFP. Does not cross-react with human cadherin. |
Affinity | n.d. |
Uniprot ID | Q90275 |
Assay compatibility | FACS, zebrafish whole mounts, western blots, ELISA |
Epitope | Binds to epitope in extracellular domain 1 of Danio rerio Cadherin 2 (zCdh2) |
Original antibody clone | SH1352-G9 |
Antibody origin | Monoclonal recombinant scFv-Fc antibody, antigen binding V regions selected from non-immunized human antibody phage display library connected to Fc part. Protein-A purified high purity protein, free of serum components |
Detection with: | Available with human Fc (mouse or rabbit on request): Detection by anti-human IgG-Fc specific secondary antibody (standard) if ordered with human Fc-part (order number ending with “H”); accordingly detection by anti-mouse IgG specific secondary antibody if ordered with mouse Fc-part (order number ending with “M”) or detection by anti-rabbit IgG specific secondary antibody if ordered with human Fc-part (order number ending with “R”). Fc of other species, or alternative formats (e.g. IgG, scFv) possible on request. |
Additional Information | Pls. find additional data in the paper cited below |
References | Russo et al. (2018) Sequence defined antibodies improve the detection of Cadherin 2 (N- Cadherin) during Zebrafish development. New Biotechnology 45, 98-112 |
ABC0203H (with human Fc-part) 100µg, lyophyllized € 399.-
ABC0203M (with mouse Fc-part) 100µg, lyophyllized request quote
ABC0203R (with rabbit Fc-part) 100µg, lyophyllized request quote
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Antigen species |
Danio rerio (Zebrafish) |
|
Specificity / Activity |
Specificity verified on cadherin knock downs and by co-location with cadherin-GFP cross-reactive in FACS on HELA and HEK293 (human) cells |
|
Affinity |
n.d. |
|
Uniprot ID |
Q90275 |
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Assay compatibility |
FACS, zebrafish whole mounts, western blots, ELISA |
|
Epitope |
Binds to core epitope: VDINGNQ in extracellular domain 1 of Danio rerio Cadherin 2 (zCdh2) |
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Original antibody clone |
SH1352-B11 |
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Antibody origin |
Monoclonal recombinant scFv-Fc antibody, antigen binding V regions selected from non-immunized human antibody phage display library connected to Fc part. Protein-A purified high purity protein, free of serum components |
|
Detection with: |
Available with human Fc (mouse or rabbit on request): Detection by anti-human IgG-Fc specific secondary antibody (standard) if ordered with human Fc-part (order number ending with “H”); accordingly detection by anti-mouse IgG specific secondary antibody if ordered with mouse Fc-part (order number ending with “M”) or detection by anti-rabbit IgG specific secondary antibody if ordered with human Fc-part (order number ending with “R”). Fc of other species, or alternative formats (e.g. IgG, scFv) possible on request. |
|
Additional Information |
Pls. find additional data in the paper cited below |
|
References |
Russo et al. (2018) Sequence defined antibodies improve the detection of Cadherin 2 (N- Cadherin) during Zebrafish development. New Biotechnology 45, 98-11Anti-Ze2 |
ABC0204H (with human Fc-part) 100µg, lyophyllized € 378.-
ABC0204M (with mouse Fc-part) 100µg, lyophyllized request quote
ABC0204R (with rabbit Fc-part) 100µg, lyophyllized request quote
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Antigen species |
Human |
|
Specificity / Activity |
Recognises SMAD3 only when SMAD3 is phosphorylated at amino acid threonine 179 in the linker region. Not reacting with SMAD3 when that T is unphosphorylated |
|
Affinity |
n.d. |
|
Uniprot ID |
P84022 |
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Assay compatibility |
ELISA, IHC and proximity ligation (visualization of individual molecules) |
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Epitope |
Phospho-EPQSNIPETPPPGYLSEDGE |
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Original antibody clone |
SH544-IIC4 |
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Antibody origin |
Monoclonal recombinant scFv-Fc antibody, antigen binding V regions selected from non-immunized human antibody phage display library connected to Fc part. Protein-A purified high purity protein, free of serum components |
|
Detection with: |
Available with human Fc (mouse or rabbit on request): Detection by anti-human IgG-Fc specific secondary antibody (standard) if ordered with human Fc-part (order number ending with “H”); accordingly detection by anti-mouse IgG specific secondary antibody if ordered with mouse Fc-part (order number ending with “M”) or detection by anti-rabbit IgG specific secondary antibody if ordered with human Fc-part (order number ending with “R”). Fc of other species, or alternative formats (e.g. IgG, scFv) possible on request. |
|
Additional Information |
Pls. find additional data in the paper cited below |
|
References |
Blokzijl, A. et al. (2016). Single chain antibodies as tools to study TGF-β regulated SMAD proteins in proximity ligation-based pharmacological screens. Molecular&Cellular Proteomics15:1848-1856) |
ABC0206H (with human Fc-part) 100µg, lyophyllized € 412.-
ABC0206M (with mouse Fc-part) 100µg, lyophyllized € 527.-
ABC0206R (with rabbit Fc-part) 100µg, lyophyllized request quote
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Antigen species |
Legionella pneumophila |
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Specificity / Activity |
whole Legionella pneumophila cells strains, in particular highly pathogenic strains Corby, Philadelphia-1 and Knoxville, lower reaction with L. pneumophila W06.1272 and Legionella micadei. No cross reactivity observed with C. freundii, E.coli, K. aerogenes, P. aeruginosa, R.planticola, C. bifermentans and C. perfringens. The LPS is still recognised after heat treatment, freeze/thaw-cycles or formaldehyde treatment of the Legionella pneumophila cells. |
|
Affinity |
n.d. |
|
Assay compatibility |
whole cell lysate western blot, cell ELISA, VANTIX Immunosensor. |
|
Antigen target |
Lipopolysaccharid (LPS) |
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Original antibody clone |
PHK121-H2 |
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Antibody origin |
Monoclonal recombinant scFv-Fc antibody, antigen binding V regions selected from non-immunized human antibody phage display library connected to Fc part. Protein-A purified high purity protein, free of serum components |
|
Detection with: |
Available with human Fc (mouse or rabbit on request): Detection by anti-human IgG-Fc specific secondary antibody (standard) if ordered with human Fc-part (order number ending with “H”); accordingly detection by anti-mouse IgG specific secondary antibody if ordered with mouse Fc-part (order number ending with “M”) or detection by anti-rabbit IgG specific secondary antibody if ordered with human Fc-part (order number ending with “R”). Fc of other species, or alternative formats (e.g. IgG, scFv) possible on request. |
|
Additional Information |
Pls. find additional data in the paper cited below |
|
References |
Kuhn, et al., (2017). Human Anti-Lipopolysaccharid (LPS) antibodies against Legionella with high species specificity. Human Antibodies 26: 29-38 |
